BASIC

Isolation and Culture of Muscle Stem Cells and Progenitor Cells

The protocol describes in detail the mechanical and enzymatic dissociation of mononucleated cells from whole limb muscles and injured tibialis anterior (TA) muscles to isolate FAPs and MuSCs. Additionally, it describes an optimized method for culturing quiescent and activated FAPs and MuSCs.
Cell Biology Developmental Biology Muscle Biology Stem Cell Biology
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