The principal mission of the Light Imaging Section is to give NIAMS scientists access to state-of-the-art equipment and to offer training and assistance in the acquisition and analysis of high quality scientific images by light microscopy. Furthermore, we use light microscopy and other techniques to elucidate poorly understood aspects of skeletal muscle cell biology, i.e. the organization of muscle microtubules and their role in muscle diseases. We also strive to apply new modalities of light microscopy to the quantitative analysis of skeletal muscle pathologies.
The Light Imaging Section is equipped for widefield and confocal microscopy, multi-photon microscopy, TIRF, high-throughput live cell imaging, and slide scanning. See full list of instruments available.
Image & Media Gallery
A High -Throughput Real-Time Imaging Technique To Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils. Gupta S, Chan DW, Zaal KJ, Kaplan MJ. J Immunol. 2018 Jan 15;200(2):869-879. doi: 10.4049/jimmunol.1700905. Epub 2017 Dec 1. PMID: 29196457
Clearing skeletal muscle with CLARITY for light microscopy imaging. Milgroom A, Ralston E. Cell Biol Int. 2016 Apr;40(4):478-83. doi: 10.1002/cbin.10578. Epub 2016 Feb 15. PMID: 26732743
A new directionality tool for assessing microtubule pattern alterations. Liu W, Ralston E. Cytoskeleton (Hoboken). 2014 Apr;71(4):230-40. doi: 10.1002/cm.21166. Epub 2014 Feb 14. PMID: 24497496
Molecular editing of cellular responses by the high-affinity receptor for IgE. Suzuki R, Leach S, Liu W, Ralston E, Scheffel J, Zhang W, Lowell CA, Rivera J. Science. 2014 Feb 28;343(6174):1021-5. doi: 10.1126/science.1246976. Epub 2014 Feb 6. PMID: 24505132
Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements. Oddoux S, Zaal KJ, Tate V, Kenea A, Nandkeolyar SA, Reid E, Liu W, Ralston E. J Cell Biol. 2013 Oct 28;203(2):205-13. doi: 10.1083/jcb.201304063. Epub 2013 Oct 21. PMID: 24145165
Quantitative evaluation of skeletal muscle defects in second harmonic generation images. Liu W, Raben N, Ralston E. J Biomed Opt. 2013 Feb;18(2):26005. doi: 0.1117/1.JBO.18.2.026005. PMID: 23377006