Light Imaging Section

Davide Randazzo, Ph.D.

    Overview

    Mission

    The principal mission of the Light Imaging Section is to give NIAMS scientists access to state-of-the-art equipment and to offer training and assistance in the acquisition and analysis of high quality scientific images by light microscopy. Furthermore, we use light microscopy and other techniques to elucidate poorly understood aspects of skeletal muscle cell biology, i.e. the organization of muscle microtubules and their role in muscle diseases. We also strive to apply new modalities of light microscopy to the quantitative analysis of skeletal muscle pathologies.

    Services

    The Light Imaging Section is equipped for widefield and confocal microscopy, multi-photon microscopy, TIRF, high-throughput live cell imaging, and slide scanning. See full list of instruments available.

    For access to instruments and more information, contact Dr. Randazzo ([email protected]) or Ms. Kenea ([email protected]).

    In the Lab with NIAMS

    Learn about the Light Imaging Section, a microscopy core with a variety of tools for image analysis.

    Core Research Facilities

    Labs at the NIAMS are supported by the following state-of-the-art facilities and services:

    Image & Media Gallery

    3D image of monoctyes
    3D View of Human Monocytes
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    Scientific Publications

    Selected Recent Publications

    Longitudinal multiomic and spatial transcriptomic profiling of lupus nephritis progression in a murine model.

    Patino-Martinez E, Hajihosseini M, Hanata N, Jiang K, Oguz C, Tandon M, Schaughency P, Randazzo D, Naz F, Dell'Orso S, Blanco LP, Pyne S, Kaplan MJ
    J Immunol.
    2026 May 14;
    215(5).
    doi: 10.1093/jimmun/vkag100
    PMID: 42152612

    A skeletal muscle atlas shows neuromuscular junction adaptations to growth and atrophy.

    Campanario S, Grima-Terrén M, Rommelfanger M, Dell' Orso S, Feng X, Cisneros A, Ramírez-Pardo I, Calls-Cobos A, Yang BA, Ko KD, García-Domínguez E, Pena-Couso L, Chou G, Zheng Y, Hah N, Randazzo D, Pérez-Garcia A, Markowitz TE, Milisenda J, Pinal-Fernandez I, Mammen A, Gómez-Cabrera MC, Serrano AL, Perdiguero E, Sartorelli V, Isern J, Muñoz-Cánoves P
    Dev Cell.
    2026 May 13;
    61(5).
    doi: 10.1016/j.devcel.2026.03.010
    PMID: 42019489

    Gain of function NOTCH4 variants disrupt angiogenesis in systemic sclerosis.

    Kaundal U, Tsou PS, Sahu M, Huang M, Boyden SE, Woodford CM, Shriner D, Stenson E, Safran SA, Zhou Y, Talley TA, Gudapati K, Zhang X, Kunishita Y, Wang J, Shah AA, Mayes MD, Doumatey AP, Bentley AR, Domsic R, Medsger TA Jr, Ramos PS, Silver RM, Steen V, Varga J, Hsu V, Saketkoo LA, Schiopu E, Gordon JK, Criswell LA, Gladue H, Derk C, Bernstein EJ, Bridges SL Jr, Shanmugam V, Chung L, Kafaja S, Jan R, Trojanowski M, Goldberg A, Korman BD, Mullikin J, Thomas JW, Dell'Orso S, Randazzo D, Adeyemo A, Remmers EF, Schwartzberg PL, Aksentijevich I, Rotimi C, Wigley FM, Wang RA, Boin F, Khanna D, Lafyatis R, Kastner DL, Gourh P
    Ann Rheum Dis.
    2026 Jul;
    85(7).
    doi: 10.1016/j.ard.2025.12.015
    PMID: 41644364

    A neutrophil-mesangial cell axis promotes glomerular injury in lupus nephritis.

    Hanata N, Carmona-Rivera C, Hoffmann V, Jiang K, Manna Z, Randazzo D, Waldman MA, Hasni SA, Kaplan MJ
    Ann Rheum Dis.
    2026 Jul;
    85(7).
    doi: 10.1016/j.ard.2025.11.011
    PMID: 41354562

    Selective requirement of glycosphingolipid synthesis for natural killer and cytotoxic T cells.

    Morrison TA, Vigee J, Tovar KA, Talley TA, Mujal AM, Kono M, Philips R, Nagashima H, Brooks SR, Dada H, Rozich I, Hudspeth K, Lau CM, Yao C, Sciumè G, Sun HW, Bonifacino JS, Kanno Y, Dustin ML, Randazzo D, Proia RL, Sun JC, Shih HY, O'Shea JJ
    Cell.
    2025 Jun 26;
    188(13).
    doi: 10.1016/j.cell.2025.04.007
    PMID: 40306279

    Biallelic human SHARPIN loss of function induces autoinflammation and immunodeficiency.

    Oda H, Manthiram K, Chavan PP, Rieser E, Veli Ö, Kaya Ö, Rauch C, Nakabo S, Kuehn HS, Swart M, Wang Y, Çelik NI, Molitor A, Ziaee V, Movahedi N, Shahrooei M, Parvaneh N, Alipour-Olyei N, Carapito R, Xu Q, Preite S, Beck DB, Chae JJ, Nehrebecky M, Ombrello AK, Hoffmann P, Romeo T, Deuitch NT, Matthíasardóttir B, Mullikin J, Komarow H, Stoddard J, Niemela J, Dobbs K, Sweeney CL, Anderton H, Lawlor KE, Yoshitomi H, Yang D, Boehm M, Davis J, Mudd P, Randazzo D, Tsai WL, Gadina M, Kaplan MJ, Toguchida J, Mayer CT, Rosenzweig SD, Notarangelo LD, Iwai K, Silke J, Schwartzberg PL, Boisson B, Casanova JL, Bahram S, Rao AP, Peltzer N, Walczak H, Lalaoui N, Aksentijevich I, Kastner DL
    Nat Immunol.
    2024 May;
    25(5).
    doi: 10.1038/s41590-024-01817-w
    PMID: 38609546

    Intravital imaging of muscle damage and response to therapy in a model of Pompe disease.

    Meena NK, Ng Y, Randazzo D, Weigert R, Puertollano R, Raben N
    Clin Transl Med.
    2024 Mar;
    14(3).
    doi: 10.1002/ctm2.1561
    PMID: 38445455

    Interrupting an IFN-γ-dependent feedback loop in the syndrome of pyogenic arthritis with pyoderma gangrenosum and acne.

    Lee W, Stone DL, Hoffmann P, Rosenzweig S, Tsai WL, Gadina M, Romeo T, Lee CR, Randazzo D, Pimpale Chavan P, Manthiram K, Canna S, Park YH, Ombrello AK, Aksentijevich I, Kastner DL, Chae JJ
    Ann Rheum Dis.
    2024 May 15;
    83(6).
    doi: 10.1136/ard-2023-225085
    PMID: 38408849

    Variant STAT4 and Response to Ruxolitinib in an Autoinflammatory Syndrome.

    Baghdassarian H, Blackstone SA, Clay OS, Philips R, Matthiasardottir B, Nehrebecky M, Hua VK, McVicar R, Liu Y, Tucker SM, Randazzo D, Deuitch N, Rosenzweig S, Mark A, Sasik R, Fisch KM, Pimpale Chavan P, Eren E, Watts NR, Ma CA, Gadina M, Schwartz DM, Sanyal A, Werner G, Murdock DR, Horita N, Chowdhury S, Dimmock D, Jepsen K, Remmers EF, Goldbach-Mansky R, Gahl WA, O'Shea JJ, Milner JD, Lewis NE, Chang J, Kastner DL, Torok K, Oda H, Putnam CD, Broderick L
    N Engl J Med.
    2023 Jun 15;
    388(24).
    doi: 10.1056/NEJMoa2202318
    PMID: 37256972

    Fostamatinib Inhibits Neutrophils Extracellular Traps Induced by COVID-19 Patient Plasma: A Potential Therapeutic.

    Strich JR, Ramos-Benitez MJ, Randazzo D, Stein SR, Babyak A, Davey RT, Suffredini AF, Childs RW, Chertow DS
    J Infect Dis.
    2021 Mar 29;
    223(6).
    doi: 10.1093/infdis/jiaa789
    PMID: 33367731

    Proteomic, biomechanical and functional analyses define neutrophil heterogeneity in systemic lupus erythematosus.

    Bashant KR, Aponte AM, Randazzo D, Rezvan Sangsari P, Wood AJ, Bibby JA, West EE, Vassallo A, Manna ZG, Playford MP, Jordan N, Hasni S, Gucek M, Kemper C, Conway Morris A, Morgan NY, Toepfner N, Guck J, Mehta NN, Chilvers ER, Summers C, Kaplan MJ
    Ann Rheum Dis.
    2021 Feb;
    80(2).
    doi: 10.1136/annrheumdis-2020-218338
    PMID: 32988843

    A High-Throughput Real-Time Imaging Technique To Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils.

    Gupta S, Chan DW, Zaal KJ, Kaplan MJ
    J Immunol.
    2018 Jan 15;
    200(2).
    doi: 10.4049/jimmunol.1700905
    PMID: 29196457

    News & Highlights

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    Last Updated: July 2026
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