Overview

Mission

The principal mission of the Light Imaging Section is to give NIAMS scientists access to state-of-the-art equipment and to offer training and assistance in the acquisition and analysis of high quality scientific images by light microscopy. Furthermore, we use light microscopy and other techniques to elucidate poorly understood aspects of skeletal muscle cell biology, i.e. the organization of muscle microtubules and their role in muscle diseases. We also strive to apply new modalities of light microscopy to the quantitative analysis of skeletal muscle pathologies.

Services

The Light Imaging Section is equipped for widefield and confocal microscopy, multi-photon microscopy, TIRF, high-throughput live cell imaging, and slide scanning. See full list of instruments available.

For access to instruments and more information, contact Dr. Randazzo (davide.randazzo@nih.gov) or Ms. Kenea (aster.kenea@nih.gov).

In the Lab with NIAMS

Learn about the Light Imaging Section, a microscopy core with a variety of tools for image analysis.

Core Research Facilities

Labs at the NIAMS are supported by the following state-of-the-art facilities and services:

Image & Media Gallery

Scientific Publications

Selected Recent Publications

Structural basis of microtubule depolymerization by the kinesin-like activity of HIV-1 Rev.

Eren E, Watts NR, Randazzo D, Palmer I, Sackett DL, Wingfield PT
Structure.
2023 Oct 5;
31(10).
doi: 10.1016/j.str.2023.07.009
PMID: 37572662

Infigratinib, a selective FGFR1-3 tyrosine kinase inhibitor, alters dentoalveolar development at high doses.

Michel ZD, Aitken SF, Glover OD, Alejandro LO, Randazzo D, Dambkowski C, Martin D, Collins MT, Somerman MJ, Chu EY
Dev Dyn.
2023 Dec;
252(12).
doi: 10.1002/dvdy.642
PMID: 37435833

AAV-mediated delivery of secreted acid α-glucosidase with enhanced uptake corrects neuromuscular pathology in Pompe mice.

Meena NK, Randazzo D, Raben N, Puertollano R
JCI Insight.
2023 Aug 22;
8(16).
doi: 10.1172/jci.insight.170199
PMID: 37463048

Polycomb Ezh1 maintains murine muscle stem cell quiescence through non-canonical regulation of Notch signaling.

Feng X, Wang AH, Juan AH, Ko KD, Jiang K, Riparini G, Ciuffoli V, Kaba A, Lopez C, Naz F, Jarnik M, Aliberti E, Hu S, Segalés J, Khateb M, Acevedo-Luna N, Randazzo D, Cheung TH, Muñoz-Cánoves P, Dell'Orso S, Sartorelli V
Dev Cell.
2023 Jun 19;
58(12).
doi: 10.1016/j.devcel.2023.04.005
PMID: 37105173

Variant STAT4 and Response to Ruxolitinib in an Autoinflammatory Syndrome.

Baghdassarian H, Blackstone SA, Clay OS, Philips R, Matthiasardottir B, Nehrebecky M, Hua VK, McVicar R, Liu Y, Tucker SM, Randazzo D, Deuitch N, Rosenzweig S, Mark A, Sasik R, Fisch KM, Pimpale Chavan P, Eren E, Watts NR, Ma CA, Gadina M, Schwartz DM, Sanyal A, Werner G, Murdock DR, Horita N, Chowdhury S, Dimmock D, Jepsen K, Remmers EF, Goldbach-Mansky R, Gahl WA, O'Shea JJ, Milner JD, Lewis NE, Chang J, Kastner DL, Torok K, Oda H, Putnam CD, Broderick L
N Engl J Med.
2023 Jun 15;
388(24).
doi: 10.1056/NEJMoa2202318
PMID: 37256972

Influenza Defective Interfering Virus Promotes Multiciliated Cell Differentiation and Reduces the Inflammatory Response in Mice.

Wang C, Honce R, Salvatore M, Chow D, Randazzo D, Yang J, Twells NM, Mahal LK, Schultz-Cherry S, Ghedin E
J Virol.
2023 Jun 29;
97(6).
doi: 10.1128/jvi.00493-23
PMID: 37255439

Fostamatinib Inhibits Neutrophils Extracellular Traps Induced by COVID-19 Patient Plasma: A Potential Therapeutic.

Strich JR, Ramos-Benitez MJ, Randazzo D, Stein SR, Babyak A, Davey RT, Suffredini AF, Childs RW, Chertow DS
J Infect Dis.
2021 Mar 29;
223(6).
doi: 10.1093/infdis/jiaa789
PMID: 33367731

Proteomic, biomechanical and functional analyses define neutrophil heterogeneity in systemic lupus erythematosus.

Bashant KR, Aponte AM, Randazzo D, Rezvan Sangsari P, Wood AJ, Bibby JA, West EE, Vassallo A, Manna ZG, Playford MP, Jordan N, Hasni S, Gucek M, Kemper C, Conway Morris A, Morgan NY, Toepfner N, Guck J, Mehta NN, Chilvers ER, Summers C, Kaplan MJ
Ann Rheum Dis.
2021 Feb;
80(2).
doi: 10.1136/annrheumdis-2020-218338
PMID: 32988843

A High-Throughput Real-Time Imaging Technique To Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils.

Gupta S, Chan DW, Zaal KJ, Kaplan MJ
J Immunol.
2018 Jan 15;
200(2).
doi: 10.4049/jimmunol.1700905
PMID: 29196457

Clearing skeletal muscle with CLARITY for light microscopy imaging.

Milgroom A, Ralston E
Cell Biol Int.
2016 Apr;
40(4).
doi: 10.1002/cbin.10578
PMID: 26732743

A new directionality tool for assessing microtubule pattern alterations.

Liu W, Ralston E
Cytoskeleton (Hoboken).
2014 Apr;
71(4).
doi: 10.1002/cm.21166
PMID: 24497496

Molecular editing of cellular responses by the high-affinity receptor for IgE.

Suzuki R, Leach S, Liu W, Ralston E, Scheffel J, Zhang W, Lowell CA, Rivera J
Science.
2014 Feb 28;
343(6174).
doi: 10.1126/science.1246976
PMID: 24505132

Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements.

Oddoux S, Zaal KJ, Tate V, Kenea A, Nandkeolyar SA, Reid E, Liu W, Ralston E
J Cell Biol.
2013 Oct 28;
203(2).
doi: 10.1083/jcb.201304063
PMID: 24145165

Quantitative evaluation of skeletal muscle defects in second harmonic generation images.

Liu W, Raben N, Ralston E
J Biomed Opt.
2013 Feb;
18(2).
doi: 10.1117/1.JBO.18.2.026005
PMID: 23377006

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