Overview

Principal Investigator

Davide Randazzo, Ph.D.

Dr. Davide Randazzo did his postdoctoral training at the University of Siena, Italy, where he characterized a mouse model knockout for the muscle protein obscurin. He moved to the U.S. in 2014, for a position as a visiting fellow at the NIAMS.

Mission

The principal mission of the Light Imaging Section is to give NIAMS scientists access to state-of-the-art equipment and to offer training and assistance in the acquisition and analysis of high quality scientific images by light microscopy. Furthermore, we use light microscopy and other techniques to elucidate poorly understood aspects of skeletal muscle cell biology, i.e. the organization of muscle microtubules and their role in muscle diseases. We also strive to apply new modalities of light microscopy to the quantitative analysis of skeletal muscle pathologies.

Services

The Light Imaging Section is equipped for widefield and confocal microscopy, multi-photon microscopy, TIRF, high-throughput live cell imaging, and slide scanning. See full list of instruments available.

For access to instruments and more information, contact Dr. Randazzo (davide.randazzo@nih.gov) or Ms. Kenea (aster.kenea@nih.gov).

Core Research Facilities

Labs at the NIAMS are supported by the following state-of-the-art facilities and services:

Image & Media Gallery

Scientific Publications

Selected Recent Publications

Fostamatinib Inhibits Neutrophils Extracellular Traps Induced by COVID-19 Patient Plasma: A Potential Therapeutic.

Strich JR, Ramos-Benitez MJ, Randazzo D, Stein SR, Babyak A, Davey RT, Suffredini AF, Childs RW, Chertow DS
J Infect Dis.
2021 Mar 29;
223(6).
doi: 10.1093/infdis/jiaa789
PMID: 33367731

Proteomic, biomechanical and functional analyses define neutrophil heterogeneity in systemic lupus erythematosus.

Bashant KR, Aponte AM, Randazzo D, Rezvan Sangsari P, Wood AJ, Bibby JA, West EE, Vassallo A, Manna ZG, Playford MP, Jordan N, Hasni S, Gucek M, Kemper C, Conway Morris A, Morgan NY, Toepfner N, Guck J, Mehta NN, Chilvers ER, Summers C, Kaplan MJ
Ann Rheum Dis.
2021 Feb;
80(2).
doi: 10.1136/annrheumdis-2020-218338
PMID: 32988843

A High-Throughput Real-Time Imaging Technique To Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils.

Gupta S, Chan DW, Zaal KJ, Kaplan MJ
J Immunol.
2018 Jan 15;
200(2).
doi: 10.4049/jimmunol.1700905
PMID: 29196457

Clearing skeletal muscle with CLARITY for light microscopy imaging.

Milgroom A, Ralston E
Cell Biol Int.
2016 Apr;
40(4).
doi: 10.1002/cbin.10578
PMID: 26732743

A new directionality tool for assessing microtubule pattern alterations.

Liu W, Ralston E
Cytoskeleton (Hoboken).
2014 Apr;
71(4).
doi: 10.1002/cm.21166
PMID: 24497496

Molecular editing of cellular responses by the high-affinity receptor for IgE.

Suzuki R, Leach S, Liu W, Ralston E, Scheffel J, Zhang W, Lowell CA, Rivera J
Science.
2014 Feb 28;
343(6174).
doi: 10.1126/science.1246976
PMID: 24505132

Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements.

Oddoux S, Zaal KJ, Tate V, Kenea A, Nandkeolyar SA, Reid E, Liu W, Ralston E
J Cell Biol.
2013 Oct 28;
203(2).
doi: 10.1083/jcb.201304063
PMID: 24145165

Quantitative evaluation of skeletal muscle defects in second harmonic generation images.

Liu W, Raben N, Ralston E
J Biomed Opt.
2013 Feb;
18(2).
doi: 10.1117/1.JBO.18.2.026005
PMID: 23377006

News & Highlights

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Last Updated: August 2021 Back to Top