Overview

The primary goal of the Flow Cytometry Section is to provide the services of state-of-the-art multi-parameter cellular analysis and cell sorting for researchers and investigators of the NIAMS IRP. The Section maintains and updates the equipment as needed, provides training for investigators, schedules sorting, and collaborates for creative solutions to scientific experiments. Particular interests include multi-parameter analysis, fluorescence resonance energy transfer, single cell sorting, and cytometric analysis of intracellular signaling events.

Services

The facility currently houses instrumentation for cell analysis and sorting.

Cell Analysis

  • (Two) BD Biosciences FACSVerse (2-lasers, 8-color with Universal Loader)
  • Beckman Coulter CyAn ADP (3-laser, 9-color analyzer)
  • BD Biosciences FACSCanto (3-laser, 8-color analyzer with HTS)
  • BD Biosciences LSR Fortessa (7-laser, 18-color, with HTS)

Cell Sorting

  • Beckman Coulter MoFlo Legacy (3-laser, 10-color, with CyClone)
  • BD Biosciences FACSAria IIIu (5-laser, 18-color, with ACDU)
  • BD Biosciences Influx (7-laser, 18-color, with ACDU and spectral analyzer)

Image & Media Gallery

Scientific Publications

 

 

Polycomb EZH2 controls self-renewal and safeguards the transcriptional identity of skeletal muscle stem cells.

Juan AH, Derfoul A, Feng X, Ryall JG, Dell'Orso S, Pasut A, Zare H, Simone JM, Rudnicki MA, Sartorelli V
Genes & development.
2011 Apr 15;
25(8).
doi: 10.1101/gad.2027911
PMID: 21498568

The gene encoding the hematopoietic stem cell regulator CCN3/NOV is under direct cytokine control through the transcription factors STAT5A/B.

Kimura A, Martin C, Robinson GW, Simone JM, Chen W, Wickre MC, O'Shea JJ, Hennighausen L
The Journal of biological chemistry.
2010 Oct 22;
285(43).
doi: 10.1074/jbc.M110.141804
PMID: 20720003

The transcription factors STAT5A/B regulate GM-CSF-mediated granulopoiesis.

Kimura A, Rieger MA, Simone JM, Chen W, Wickre MC, Zhu BM, Hoppe PS, O'Shea JJ, Schroeder T, Hennighausen L
Blood.
2009 Nov 19;
114(21).
doi: 10.1182/blood-2009-04-216390
PMID: 19779039

Latest News

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Contact Us

Ronnie Gladney

IRP Support Staff
9000 Rockville Pike
Bldg 10, Room 10N311
Bethesda MD 20892
Last Updated: May 2020