Overview

The Flow Cytometry Section provides state-of-the-art high-parameter cell analysis and sorting services to support NIAMS IRP investigators in their pursuit of the Institute’s mission. We provide sorting services, maintain and update the equipment, train instrument users, and collaborate on creative solutions to scientific questions. Our interests include single-cell multiomics, high-parameter data reduction analysis, and cytometric analysis of intracellular signaling events.

Instrumentation

The facility currently houses a variety of state-of-the-art instrumentation for cell analysis and sorting.

Cell Analysis

  • BD FACSCanto RUO: 4-laser, 14-color analyzer with HTS (high throughput sampler)
  • BD LSR Fortessa SORP: 7-laser, 18-color analyzer with HTS BD FACSCelesta: 4-laser, 15-color analyzer
  • BD FACSymphony A5: 5-laser, 24-color analyzer with HTS
  • Cytek Aurora: 5-laser, 35-color analyzer with autoloader
  • BD FACSymphony A3: 5-laser, 24-color analyzer with HTS (Q3 2020)
  • ThermoFisher Attune NxT: 4-laser, 14-color analyzer with autosampler (Q3 2020)

Cell Sorting

  • BD FACSAria IIIu: 5-laser, 18-color cell sorter with ACDU (automated cell deposition unit)
  • BD FACSAria Fusion: 5-laser, 18-color cell sorter with ACDU and biosafety cabinet
  • BD Influx: 7-laser, 18-color cell sorter with ACDU and biosafety cabinet
  • BD FACSymphony S6: 5-laser, 30-color analyzer with ACDU and biosafety cabinet (Q1 2021)
  • BD FACSMelody: 3-laser, 9-color cell sorter with biosafety cabinet (Q1 2021)

Core Research Facilities

Labs at the NIAMS are supported by the following state-of-the-art facilities and services:

Staff

Senior Research Assistant
301-594-6191
Biologist
301-594-6169
Staff Scientist
301-435-5513

Image & Media Gallery

Scientific Publications

Selected Recent Publications

Polycomb EZH2 controls self-renewal and safeguards the transcriptional identity of skeletal muscle stem cells.

Juan AH, Derfoul A, Feng X, Ryall JG, Dell'Orso S, Pasut A, Zare H, Simone JM, Rudnicki MA, Sartorelli V
Genes Dev.
2011 Apr 15;
25(8).
doi: 10.1101/gad.2027911
PMID: 21498568

The gene encoding the hematopoietic stem cell regulator CCN3/NOV is under direct cytokine control through the transcription factors STAT5A/B.

Kimura 木村丹香子 A, Martin C, Robinson GW, Simone JM, Chen W, Wickre MC, O'Shea JJ, Hennighausen L
J Biol Chem.
2010 Oct 22;
285(43).
doi: 10.1074/jbc.M110.141804
PMID: 20720003

The transcription factors STAT5A/B regulate GM-CSF-mediated granulopoiesis.

Kimura A, Rieger MA, Simone JM, Chen W, Wickre MC, Zhu BM, Hoppe PS, O'Shea JJ, Schroeder T, Hennighausen L
Blood.
2009 Nov 19;
114(21).
doi: 10.1182/blood-2009-04-216390
PMID: 19779039

News & Highlights

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Last Updated: May 2021 Back to Top