Flow Cytometry Section

James Simone

Overview

Senior Research Assistant

The Flow Cytometry Section provides state-of-the-art high-parameter cell analysis and sorting services to support NIAMS IRP investigators in their pursuit of the Institute’s mission. We provide sorting services, maintain and update the equipment, train instrument users, and collaborate on creative solutions to scientific questions. Our interests include single-cell multiomics, high-parameter data reduction analysis, and cytometric analysis of intracellular signaling events.

Instrumentation

The facility currently houses a variety of state-of-the-art instrumentation for cell analysis and sorting.

Cell Analysis

  • BD LSR Fortessa SORP: 7-laser, 18-color analyzer with HTS
  • BD FACSymphony A5: 5-laser, 24-color analyzer with HTS
  • Cytek Aurora Research: 5-laser, full-spectrum 40-color analyzer with autoloader
  • Cytek Aurora Clinical: 5-laser, full-spectrum 40-color analyzer with autoloader
  • BD FACSymphony A3: 5-laser, 23-color analyzer with HTS and FACSFlow Supply System
  • BD FACSCelesta​​ SORP: 4-laser, 15-color analyzer
  • ThermoFisher Attune NxT: 4-laser, 14-color analyzer with autosampler

Cell Sorting

  • BD FACSAria Fusion: 5-laser, 18-color cell sorter with ACDU, biosafety cabinet
  • BD FACSMelody: 3-laser, 9-color, cell sorter with ADCU, biosafety cabinet
  • Cytek Aurora CS: 5-laser, full spectrum 40-color cell sorter, CDU, biosafety cabinet
  • BD FACSDiscover S8: 5-laser, full-spectrum 50-color cell sorter with imaging capability, ADCU, biosafety cabinet

Contact Us

In the Lab with NIAMS

Learn about the Flow Cytometry Section, which offers cell sorting platforms to help researchers isolate and analyze cell populations that contribute to human disease.

Core Research Facilities

Labs at the NIAMS are supported by the following state-of-the-art facilities and services:

Staff

Senior Research Assistant
Jeffrey T. Lay
Biologist
Staff Scientist

Image & Media Gallery

Scientific Publications

Selected Recent Publications

FACS-isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle.

Riparini G, Simone JM, Sartorelli V
J Vis Exp.
2022 Jun 8;
(184).
doi: 10.3791/63983
PMID: 35758697

Polycomb EZH2 controls self-renewal and safeguards the transcriptional identity of skeletal muscle stem cells.

Juan AH, Derfoul A, Feng X, Ryall JG, Dell'Orso S, Pasut A, Zare H, Simone JM, Rudnicki MA, Sartorelli V
Genes Dev.
2011 Apr 15;
25(8).
doi: 10.1101/gad.2027911
PMID: 21498568

The gene encoding the hematopoietic stem cell regulator CCN3/NOV is under direct cytokine control through the transcription factors STAT5A/B.

Kimura 木村丹香子 A, Martin C, Robinson GW, Simone JM, Chen W, Wickre MC, O'Shea JJ, Hennighausen L
J Biol Chem.
2010 Oct 22;
285(43).
doi: 10.1074/jbc.M110.141804
PMID: 20720003

The transcription factors STAT5A/B regulate GM-CSF-mediated granulopoiesis.

Kimura A, Rieger MA, Simone JM, Chen W, Wickre MC, Zhu BM, Hoppe PS, O'Shea JJ, Schroeder T, Hennighausen L
Blood.
2009 Nov 19;
114(21).
doi: 10.1182/blood-2009-04-216390
PMID: 19779039

News & Highlights

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Last Updated: November 2024