Overview

The mission of the Genomic Technology Section is to help NIAMS investigators solve highly diverse biological and biomedical research questions by providing a diverse portfolio of sequencing services as well as consultation and training for researchers with specific needs or challenging problems. The core is a state-of-the-art laboratory facility equipped with the latest DNA sequencing and library preparation technologies. A wide array of sample preparation methods enable the use of diverse applications, including: Whole-genome sequencing, RNA-Seq, small RNA discovery, methylation profiling, genome-wide protein-DNA interaction analysis (ChIP-Seq) and ATAC-Seq, exome sequencing, amplicon sequencing, Single Cells RNA-seq, Single Cells ATAC-Seq and Single Cell western.

Scientific Publications

Dysregulated neutrophil responses and neutrophil extracellular trap formation and degradation in PAPA syndrome. Mistry P, Carmona-Rivera C, Ombrello AK, Hoffmann P, Seto NL, Jones A, Stone DL, Naz F, Carlucci P, Dell'Orso S, Gutierrez-Cruz G, Sun HW, Kastner DL, Aksentijevich I, Kaplan MJ. Ann Rheum Dis. 2018 Dec;77(12):1825-1833. doi: 10.1136/annrheumdis-2018-213746. Epub 2018 Aug 21. PMID: 30131320

A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans. Tsai PF, Dell'Orso S, Rodriguez J, Vivanco KO, Ko KD, Jiang K, Juan AH, Sarshad AA, Vian L, Tran M, Wangsa D, Wang AH, Perovanovic J, Anastasakis D, Ralston E, Ried T, Sun HW, Hafner M, Larson DR, Sartorelli V. Mol Cell. 2018 Jul 5;71(1):129-141.e8. doi: 10.1016/j.molcel.2018.06.008. PMID: 29979962

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies. Feng X, Naz F, Juan AH, Dell'Orso S, Sartorelli V. J Vis Exp. 2018 Apr 19;(134). doi: 10.3791/57212. PMID: 29733324

Neutrophil subsets and their gene signature associate with vascular inflammation and coronary atherosclerosis in lupus. Carlucci PM, Purmalek MM, Dey AK, Temesgen-Oyelakin Y, Sakhardande S, Joshi AA, Lerman JB, Fike A, Davis M, Chung JH, Playford MP, Naqi M, Mistry P, Gutierrez-Cruz G, Dell'Orso S, Naz F, Salahuddin T, Natarajan B, Manna Z, Tsai WL, Gupta S, Grayson P, Teague H, Chen MY, Sun HW, Hasni S, Mehta NN, Kaplan MJ. JCI Insight. 2018 Apr 19;3(8). pii: 99276. doi: 10.1172/jci.insight.99276. [Epub ahead of print] PMID: 29669944

Aberrant tRNA processing causes an autoinflammatory syndrome responsive to TNF inhibitors. Giannelou A, Wang H, Zhou Q, Park YH, Abu-Asab MS, Ylaya K, Stone DL, Sediva A, Sleiman R, Sramkova L, Bhatla D, Serti E, Tsai WL, Yang D, Bishop K, Carrington B, Pei W, Deuitch N, Brooks S, Edwan JH, Joshi S, Prader S, Kaiser D, Owen WC, Sonbul AA, Zhang Y, Niemela JE, Burgess SM, Boehm M, Rehermann B, Chae J, Quezado MM, Ombrello AK, Buckley RH, Grom AA, Remmers EF, Pachlopnik JM, Su HC, Gutierrez-Cruz G, Hewitt SM, Sood R, Risma K, Calvo KR, Rosenzweig SD, Gadina M, Hafner M, Sun HW, Kastner DL, Aksentijevich I. Ann Rheum Dis. 2018 Apr;77(4):612-619. doi: 10.1136/annrheumdis-2017-212401. Epub 2018 Jan 22. PMID: 29358286

The Elongation Factor Spt6 Maintains ESC Pluripotency by Controlling Super-Enhancers and Counteracting Polycomb Proteins. Wang AH, Juan AH, Ko KD, Tsai PF, Zare H, Dell'Orso S, Sartorelli V. Mol Cell. 2017 Oct 19;68(2):398-413.e6. doi: 10.1016/j.molcel.2017.09.016. Epub 2017 Oct 12. PMID: 29033324

Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells. Juan AH, Wang S, Ko KD, Zare H, Tsai PF, Feng X, Vivanco KO, Ascoli AM, Gutierrez-Cruz G, Krebs J, Sidoli S, Knight AL, Pedersen RA, Garcia BA, Casellas R, Zou J, Sartorelli V. Cell Rep. 2016 Oct 25;17(5):1369-1382. doi: 10.1016/j.celrep.2016.09.087. PMID: 27783950

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Last Updated: July 2019