This study investigated the molecular and epigenetic mechanisms that lead to the formation of muscle and neuronal cells from embryonic stem cells (ESCs) at the single-cell level. The report identifies regions of the genome called enhancers that control genes critical for muscle and neuronal cell differentiation.

What is exciting about this article?

This study identified the underlying mechanisms that regulate the differentiation of ESCs into muscle and neuronal cells. These findings have the potential to provide important insights into muscle regenerative medicine through “in-a-dish” modeling of muscle disease, drug screening, and cell therapeutics.

How does this fit into the larger NIAMS portfolio?

This paper provides valuable data for understanding how muscle cells are specified and committed, and for generating muscle progenitors starting from ESCs or induced pluripotent stem cells.

Grant support


Research Areas:

Computational Biology Developmental Biology Genetics and Genomics Muscle Biology Stem Cell Biology


Transcriptomics, regulatory syntax, and enhancer identification in mesoderm-induced ESCs at single-cell resolution.

Khateb M, Perovanovic J, Ko KD, Jiang K, Feng X, Acevedo-Luna N, Chal J, Ciuffoli V, Genzor P, Simone J, Haase AD, Pourquié O, Dell'Orso S, Sartorelli V
Cell Rep.
2022 Aug 16;
doi: 10.1016/j.celrep.2022.111219
PMID: 35977485

Research reported in this publication was supported by the Intramural Research Program of the NIHʼs National Institute of Arthritis and Musculoskeletal and Skin Diseases.