James Simone

Senior Research Assistant

Section Lead, Flow Cytometry Section

Summary

James Simone first experienced the emerging field of flow cytometry in 1985 at Columbia Presbyterian Medical Center, initially in the Immunopathology Department and then at the Comprehensive Cancer Center.

In 1990, he was hired as the operator and manager of the research flow core in the Department of Immunology of SUNY at Stony Brook, using the latest instruments from BD Biosciences. From 1993 to 1997, he managed the flow cytometry laboratory and was an IT systems administrator for a startup company, Ingenex, Inc., which researched HIV and cancer. In 1997, Pharmaceutical Product Development Inc. acquired the group, and Mr. Simone continued to oversee the facility until 2003.

In September 2003, Mr. Simone became flow core manager/section leader at NIAMS, NIH. Over the past two decades, he brought in state-of-the-art flow cytometry equipment and assisted in implementing novel cytometry techniques to further the NIAMS mission.

Research Statement

As a flow core manager, Mr. Simone and his team support all facets of research at NIAMS. He is most interested in how dysregulation of the immune system can result in autoimmunity diseases such as systemic lupus erythematosus and Crohn’s disease. 

Scientific Publications

Transcriptomics, regulatory syntax, and enhancer identification in mesoderm-induced ESCs at single-cell resolution.

Khateb M, Perovanovic J, Ko KD, Jiang K, Feng X, Acevedo-Luna N, Chal J, Ciuffoli V, Genzor P, Simone J, Haase AD, Pourquié O, Dell'Orso S, Sartorelli V
Cell Rep.
2022 Aug 16;
40(7).
doi: 10.1016/j.celrep.2022.111219
PMID: 35977485

FACS-isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle.

Riparini G, Simone JM, Sartorelli V
J Vis Exp.
2022 Jun 8;
(184).
doi: 10.3791/63983
PMID: 35758697

Polycomb EZH2 controls self-renewal and safeguards the transcriptional identity of skeletal muscle stem cells.

Juan AH, Derfoul A, Feng X, Ryall JG, Dell'Orso S, Pasut A, Zare H, Simone JM, Rudnicki MA, Sartorelli V
Genes Dev.
2011 Apr 15;
25(8).
doi: 10.1101/gad.2027911
PMID: 21498568

The gene encoding the hematopoietic stem cell regulator CCN3/NOV is under direct cytokine control through the transcription factors STAT5A/B.

Kimura 木村丹香子 A, Martin C, Robinson GW, Simone JM, Chen W, Wickre MC, O'Shea JJ, Hennighausen L
J Biol Chem.
2010 Oct 22;
285(43).
doi: 10.1074/jbc.M110.141804
PMID: 20720003

The transcription factors STAT5A/B regulate GM-CSF-mediated granulopoiesis.

Kimura A, Rieger MA, Simone JM, Chen W, Wickre MC, Zhu BM, Hoppe PS, O'Shea JJ, Schroeder T, Hennighausen L
Blood.
2009 Nov 19;
114(21).
doi: 10.1182/blood-2009-04-216390
PMID: 19779039

Measurement of two caspase activities simultaneously in living cells by a novel dual FRET fluorescent indicator probe.

Wu X, Simone J, Hewgill D, Siegel R, Lipsky PE, He L
Cytometry A.
2006 Jun;
69(6).

Education

SUNY at Stony Brook 
Immunology (1991)

Columbia University 
Foreign Language (1989) 

Cornell University 
Microbiology/Immunology (1976-1978) 

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