Manasi Gadkari, M.S., received her bachelor’s degree in pharmacy from the University of Pune in India and her master’s degree in biotechnology from the University of Houston-Clear Lake. From 2011 to 2015, she was a Research Assistant in the Department of Pediatrics and the Department of Molecular and Human Genetics at Baylor College of Medicine (BCM) in Houston. As part of the Laboratory for Translational Genomics at BCM, she developed extensive expertise in next-generation sequencing technologies. From 2015 to 2019, she was a Research Associate at the Laboratory of Immune System Biology at the NIAID. There, she gained experience in the purification and culture of primary human cells, flow cytometry, molecular imaging for live cell tracking, single-molecule RNA FISH, and high-content imaging. She then joined the NIAMS in 2019.

Research Statement

Manasi’s research focuses on the application of advanced genomic and other technologies to the study of the effects of glucocorticoids on human immune and non-immune cells. Her research involves in vitro studies in primary human cells and in vivo studies with rhesus macaques, healthy human subjects, and patients with genetic disorders. She provides specialized scientific support, including developing assays and screening methods, training new lab members, and laboratory management. 

Scientific Publications

High-throughput imaging of mRNA at the single-cell level in human primary immune cells.

Gadkari M, Sun J, Carcamo A, Alessi H, Hu Z, Fraser IDC, Pegoraro G, Franco LM
2022 Sep;
doi: 10.1261/rna.079239.122
PMID: 35764396

GCgx: transcriptome-wide exploration of the response to glucocorticoids.

Cao Q, Boo Irizarry Y, Yazhuk S, Tran T, Gadkari M, Franco LM
J Mol Endocrinol.
2021 Dec 10;
doi: 10.1530/JME-21-0107
PMID: 34787097

Single-Cell Analysis Reveals the Range of Transcriptional States of Circulating Human Neutrophils.

Wigerblad G, Cao Q, Brooks S, Naz F, Gadkari M, Jiang K, Gupta S, O'Neil L, Dell'Orso S, Kaplan MJ, Franco LM
J Immunol.
2022 Aug 15;
doi: 10.4049/jimmunol.2200154
PMID: 35858733

Glucocorticoid-induced eosinopenia results from CXCR4-dependent bone marrow migration.

Hong SG, Sato N, Legrand F, Gadkari M, Makiya M, Stokes K, Howe KN, Yu SJ, Linde NS, Clevenger RR, Hunt T, Hu Z, Choyke PL, Dunbar CE, Klion AD, Franco LM
2020 Dec 3;
doi: 10.1182/blood.2020005161
PMID: 32659786

Sex differences in neutrophil biology modulate response to type I interferons and immunometabolism.

Gupta S, Nakabo S, Blanco LP, O'Neil LJ, Wigerblad G, Goel RR, Mistry P, Jiang K, Carmona-Rivera C, Chan DW, Wang X, Pedersen HL, Gadkari M, Howe KN, Naz F, Dell'Orso S, Hasni SA, Dempsey C, Buscetta A, Frischmeyer-Guerrerio PA, Kruszka P, Muenke M, Franco LM, Sun HW, Kaplan MJ
Proc Natl Acad Sci U S A.
2020 Jul 14;
doi: 10.1073/pnas.2003603117
PMID: 32601182

A Deep Learning Pipeline for Nucleus Segmentation.

Zaki G, Gudla PR, Lee K, Kim J, Ozbun L, Shachar S, Gadkari M, Sun J, Fraser IDC, Franco LM, Misteli T, Pegoraro G
Cytometry A.
2020 Dec;
doi: 10.1002/cyto.a.24257
PMID: 33141508

Immune regulation by glucocorticoids can be linked to cell type-dependent transcriptional responses.

Franco LM, Gadkari M, Howe KN, Sun J, Kardava L, Kumar P, Kumari S, Hu Z, Fraser IDC, Moir S, Tsang JS, Germain RN
J Exp Med.
2019 Feb 4;
doi: 10.1084/jem.20180595
PMID: 30674564

Transcript- and protein-level analyses of the response of human eosinophils to glucocorticoids.

Gadkari M, Makiya MA, Legrand F, Stokes K, Brown T, Howe K, Khoury P, Hu Z, Klion A, Franco LM
Sci Data.
2018 Dec 4;
doi: 10.1038/sdata.2018.275
PMID: 30512017

Glucocorticoid-induced eosinopenia in humans can be linked to early transcriptional events.

Khoury P, Stokes K, Gadkari M, Makiya MA, Legrand F, Hu Z, Klion A, Franco LM
2018 Oct;
doi: 10.1111/all.13497
PMID: 29885264

Syndromic congenital myelofibrosis associated with a loss-of-function variant in RBSN.

Magoulas PL, Shchelochkov OA, Bainbridge MN, Ben-Shachar S, Yatsenko S, Potocki L, Lewis RA, Searby C, Marcogliese AN, Elghetany MT, Zapata G, Hernández PP, Gadkari M, Einhaus D, Muzny DM, Gibbs RA, Bertuch AA, Scott DA, Corvera S, Franco LM
2018 Aug 9;
doi: 10.1182/blood-2017-12-824433
PMID: 29784638


University of Houston-Clear Lake, Houston, TX, U.S.A.
M.S., Biotechnology (2011)

University of Pune, India
B. Pharm (2009)


Research Associate
Laboratory of Immune System Biology, NIH/NIAID, Bethesda, MD (2015-2019)

Research Assistant
Department of Molecular and Human Genetics, Baylor College of Medicine, Research Center, Houston, TX (2014-2015)

Research Assistant
Department of Pediatrics, Baylor College of Medicine, USDA/ARS Children's Nutrition Research Center, Houston, TX (2011-2013)

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