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Light Imaging Section
The mission of the Light Imaging Section is to give NIAMS scientists access to state-of-the-art light imaging equipment and to offer training and assistance at all stages of the acquisition and analysis of images with various techniques of light microscopy.
Our research focuses on the use of light microscopy techniques to elucidate poorly understood aspects of skeletal muscle cell biology, i.e. the organization of microtubules and their associated subcellular organelles, particularly the Golgi complex. We also strive to apply new modalities of light microscopy to the quantitative analysis of skeletal muscle pathologies.
Raben N, Wong A, Ralston E, Myerowitz R. Autophagy and mitochondria in Pompe disease: nothing is so new as what has long been forgotten. Am J Med Genet C Semin Med Genet. 2012 Feb 15;160(1):13-21. doi: 10.1002/ajmg.c.31317
Zaal KJ, Reid E, Mousavi K, Zhang T, Mehta A, Bugnard E, Sartorelli V, Ralston E. Who needs microtubules? Myogenic reorganization of MTOC, Golgi complex and ER exit sites persists despite lack of normal microtubule tracks. PLoS One. 2011;6(12):e29057. Epub 2011 Dec 27.
Raben N, Schreiner C, Baum R, Takikita S, Xu S, Xie T, Myerowitz R, Komatsu M, Van Der Meulen JH, Nagaraju K, Ralston E, Plotz PH. Suppression of autophagy permits successful enzyme replacement therapy in a lysosomal storage disorder-murine Pompe disease. Autophagy. 2010 Nov 16;6(8):1078-89.
Raben N, Ralston E, Chien YH, Baum R, Schreiner C, Hwu WL, Zaal KJ, Plotz PH. Differences in the predominance of lysosomal and autophagic pathologies between infants and adults with Pompe disease: implications for therapy. Mol Genet Metab. 2010 Dec;101(4):324-31
Takikita S., Schreiner C., Baum R., Xie T., Ralston E., Plotz P.H., Raben N. (2010) Fiber type conversion by PGC-1α activates lysosomal and autophagosomal biogenesis in both normal and Pompe skeletal muscle. PLoS ONE. 2010 Dec 13;5(12):e15239.
Zhang T., Zaal, K.J.M., Sheridan, J., Mehta, A., Gundersen, G., and Ralston, E. (2009) The Microtubule Plus-End Binding Protein EB1 is Necessary for Muscle Cell Differentiation, Elongation, and Fusion. J. Cell Sci.2009 May 1;122(Pt 9):1401-9.
Raben N, Baum R, Schreiner C, Takikita S, Mizushima N, Ralston E, Plotz P. When more is less: excess and deficiency of autophagy coexist in skeletal muscle in Pompe disease. Autophagy. 2009 Jan 1;5(1):111-3.See extended list of publications
Reviewed December 14, 2012