Light Imaging Section

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Evelyn Ralston, Ph.D.
Chief, Light Imaging Section
Office of Science and Technology (OST)
Phone: (301) 496-6164
Fax: (301) 402-3417
E-mail: ralstone@mail.nih.gov

Overview

The mission of the Light Imaging Section is to give NIAMS scientists access to state-of-the-art light imaging equipment and to offer training and assistance at all stages of the acquisition and analysis of images with various techniques of light microscopy.

Our research focuses on the use of light microscopy techniques to elucidate poorly understood aspects of skeletal muscle cell biology, i.e. the organization of microtubules and their associated subcellular organelles, particularly the Golgi complex. We also strive to apply new modalities of light microscopy to the quantitative analysis of skeletal muscle pathologies.


Selected Publications

Oddoux S, Zaal KJ, Tate V, Kenea A, Nandkeolyar SA, Reid E, Liu W, Ralston E. Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements. J Cell Biol. 2013 Oct 28;203(2):205-13. doi: 10.1083/jcb.201304063. Epub 2013 Oct 21. PubMed Icon

Liu W, Raben N, Ralston E. Quantitative evaluation of skeletal muscle defects in second harmonic generation images. J Biomed Opt. 2013 Feb 1;18(2):26005. doi: 10.1117/1.JBO.18.2.026005. PubMed Icon

Raben N, Wong A, Ralston E, Myerowitz R. Autophagy and mitochondria in Pompe disease: nothing is so new as what has long been forgotten. Am J Med Genet C Semin Med Genet. 2012 Feb 15;160(1):13-21. doi: 10.1002/ajmg.c.31317 PubMed Icon

Zaal KJ, Reid E, Mousavi K, Zhang T, Mehta A, Bugnard E, Sartorelli V, Ralston E. Who needs microtubules? Myogenic reorganization of MTOC, Golgi complex and ER exit sites persists despite lack of normal microtubule tracks. PLoS One. 2011;6(12):e29057. Epub 2011 Dec 27. PubMed Icon

Raben N, Schreiner C, Baum R, Takikita S, Xu S, Xie T, Myerowitz R, Komatsu M, Van Der Meulen JH, Nagaraju K, Ralston E, Plotz PH. Suppression of autophagy permits successful enzyme replacement therapy in a lysosomal storage disorder-murine Pompe disease. Autophagy. 2010 Nov 16;6(8):1078-89. PubMed Icon

Raben N, Ralston E, Chien YH, Baum R, Schreiner C, Hwu WL, Zaal KJ, Plotz PH. Differences in the predominance of lysosomal and autophagic pathologies between infants and adults with Pompe disease: implications for therapy. Mol Genet Metab. 2010 Dec;101(4):324-31 PubMed Icon

Prins, K. W., Humston, J. L., Mehta, A., Tate, V., Ralston, E., & Ervasti, J. M. (2009) Dystrophin is a microtubule-associated protein. J. Cell Biol. 186, 363-9. PubMed Icon

Zhang T., Zaal, K.J.M., Sheridan, J., Mehta, A., Gundersen, G., and Ralston, E. (2009) The Microtubule Plus-End Binding Protein EB1 is Necessary for Muscle Cell Differentiation, Elongation, and Fusion. J. Cell Sci.2009 May 1;122(Pt 9):1401-9. PubMed Icon

Raben N, Baum R, Schreiner C, Takikita S, Mizushima N, Ralston E, Plotz P. When more is less: excess and deficiency of autophagy coexist in skeletal muscle in Pompe disease. Autophagy. 2009 Jan 1;5(1):111-3. PubMed Icon

See extended list of publications

 

Reviewed December 14, 2012