RNA Molecular Biology Group

Markus Hafner, Ph.D.
RNA Molecular Biology Group
Laboratory of Muscle Stem Cells and Gene Regulation
Building 50, Room 1154
Phone: 301-402-6956
E-mail: markus.hafner@nih.gov

The Hafner group focuses on dissecting the composition of ribonucleoproteins (RNPs) involved in cellular RNA transport and control of RNA stability. This is a prerequisite for understanding the consequences of dysregulation and/or mutation of RNA-binding proteins (RBPs), and/or their target RNA-binding sites in disease.


Selected Publications

Lu YC, Chang SH, Hafner M, Li X, Tuschl T, Elemento O, Hla T. ELAVL1 modulates transcriptome-wide miRNA binding in murine macrophages. Cell Rep. 2014 Dec 24;9(6):2330-43. doi: 10.1016/j.celrep.2014.11.030. Epub 2014 Dec 18. PubMed Icon

Gerstberger, S., Hafner, M., Tuschl T. A census of human RNA binding proteins. Nat Rev Genet. 2014 Dec;15(12):829-45. doi: 10.1038/nrg3813. Epub 2014 Nov 4. PubMed Icon

Yoon, J.-H, De, S., Srikantan S., Abdelmohsen, K., Grammatikakis, I., Kim, J., Kim, K.M., , Hafner, M., and Gorospe, M. PAR-CLIP analysis uncovers AUF1 impact on target RNA fate and genome integrity. Nat Commun. 2014 Nov 4;5:5248. doi: 10.1038/ncomms6248. PubMed Icon

Karaca, E., Weitzer, S., Pehlivan, D., Shiraishi, H., Gogakos, T., Hanada, T., Jhangiani, S.N., Wiszniewski, W., Withers, M., Campbell, I.M., , Hafner M., et al. Human CLP1 Mutations Alter tRNA Biogenesis, Affecting Both Peripheral and Central Nervous System Function. Cell. 2014 Apr 24;157(3):636-50. doi: 10.1016/j.cell.2014.02.058. PubMed Icon

Wang X, Wang HK, Li Y, Hafner M, Banerjee NS, Tang S, Briskin D, Meyers C, Chow LT, Xie X, Tuschl T, Zheng ZM. microRNAs are biomarkers of oncogenic human papillomavirus infections. Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4262-7. doi: 10.1073/pnas.1401430111. Epub 2014 Mar 3. PubMed Icon

Mukherjee N, Jacobs NC, Hafner M, Kennington EA, Nusbaum JD, Tuschl T, Blackshear PJ, Ohler U. Global target mRNA specification and regulation by the RNA-binding protein ZFP36. Genome Biol. 2014 Jan 8;15(1):R12. PubMed Icon

Hafner M, Max KE, Bandaru P, Morozov P, Gerstberger S, Brown M, Molina H, Tuschl T. Identification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition. RNA. 2013 May;19(5):613-26. doi: 10.1261/rna.036491.112. PubMed Icon

Teplova M, Hafner M, Teplov D, Essig K, Tuschl T, Patel DJ. Structure-function studies of STAR family Quaking proteins bound to their in vivo RNA target sites. Genes Dev. 2013 Apr 15;27(8):928-40. doi: 10.1101/gad.216531.113. PubMed Icon

Ascano M Jr, Mukherjee N, Bandaru P, Miller JB, Nusbaum JD, Corcoran DL, Langlois C, Munschauer M, Dewell S, Hafner M, Williams Z, Ohler U, Tuschl T. FMRP targets distinct mRNA sequence elements to regulate protein expression. Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. PubMed Icon

Hafner M, Renwick N, Farazi TA, Mihailović A, Pena JT, Tuschl T. Barcoded cDNA library preparation for small RNA profiling by next-generation sequencing. Methods. 2012 Oct;58(2):164-70. doi: 10.1016/j.ymeth.2012.07.030. PubMed Icon

See extended list of publications

 

Updated January 23, 2014