RNA Molecular Biology Group

Markus Hafner, Ph.D.
RNA Molecular Biology Group
Laboratory of Muscle Stem Cells and Gene Regulation
Building 50, Room 1154
Phone: 301-402-6956
E-mail: markus.hafner@nih.gov

The Hafner group focuses on dissecting the composition of ribonucleoproteins (RNPs) involved in cellular RNA transport and control of RNA stability. This is a prerequisite for understanding the consequences of dysregulation and/or mutation of RNA-binding proteins (RBPs), and/or their target RNA-binding sites in disease.


Selected Publications

Hafner M, Max KE, Bandaru P, Morozov P, Gerstberger S, Brown M, Molina H, Tuschl T. Identification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition. RNA. 2013 May;19(5):613-26. doi: 10.1261/rna.036491.112. PubMed Icon

Teplova M, Hafner M, Teplov D, Essig K, Tuschl T, Patel DJ. Structure-function studies of STAR family Quaking proteins bound to their in vivo RNA target sites. Genes Dev. 2013 Apr 15;27(8):928-40. doi: 10.1101/gad.216531.113. PubMed Icon

Ascano M Jr, Mukherjee N, Bandaru P, Miller JB, Nusbaum JD, Corcoran DL, Langlois C, Munschauer M, Dewell S, Hafner M, Williams Z, Ohler U, Tuschl T. FMRP targets distinct mRNA sequence elements to regulate protein expression. Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. PubMed Icon

Hafner M, Renwick N, Farazi TA, Mihailović A, Pena JT, Tuschl T. Barcoded cDNA library preparation for small RNA profiling by next-generation sequencing. Methods. 2012 Oct;58(2):164-70. doi: 10.1016/j.ymeth.2012.07.030. PubMed Icon

Hafner M, Lianoglou S, Tuschl T, Betel D. Genome-wide identification of miRNA targets by PAR-CLIP. Methods. 2012 Oct;58(2):94-105. doi: 10.1016/j.ymeth.2012.08.006. PubMed Icon

Ascano M, Hafner M, Cekan P, Gerstberger S, Tuschl T. Identification of RNA-protein interaction networks using PAR-CLIP. Wiley Interdiscip Rev RNA. 2012 Mar-Apr;3(2):159-77. doi: 10.1002/wrna.1103. PubMed Icon

Gottwein E, Corcoran DL, Mukherjee N, Skalsky RL, Hafner M, Nusbaum JD, Shamulailatpam P, Love CL, Dave SS, Tuschl T, Ohler U, Cullen BR. Viral microRNA targetome of KSHV-infected primary effusion lymphoma cell lines. Cell Host Microbe. 2011 Nov 17;10(5):515-26. doi: 10.1016/j.chom.2011.09.012. PubMed Icon

Hafner M, Renwick N, Brown M, Mihailović A, Holoch D, Lin C, Pena JT, Nusbaum JD, Morozov P, Ludwig J, Ojo T, Luo S, Schroth G, Tuschl T. RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries. RNA. 2011 Sep;17(9):1697-712. doi: 10.1261/rna.2799511 PubMed Icon

Hafner M, Landthaler M, Burger L, Khorshid M, Hausser J, Berninger P, Rothballer A, Ascano M Jr, Jungkamp AC, Munschauer M, Ulrich A, Wardle GS, Dewell S, Zavolan M, Tuschl T. Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP. Cell. 2010 Apr 2;141(1):129-41. doi: 10.1016/j.cell.2010.03.009. PubMed Icon

Hafner M, Schmitz A, Grüne I, Srivatsan SG, Paul B, Kolanus W, Quast T, Kremmer E, Bauer I, Famulok M. Inhibition of cytohesins by SecinH3 leads to hepatic insulin resistance. Nature. 2006 Dec 14;444(7121):941-4. PubMed Icon

See extended list of publications

 

Updated January 23, 2014