Research Statement

During my PhD I was focused on studying the epigenetic and transcriptional regulatory circuitries that modulate hematopoietic cell differentiation pathways with particular attention to the key role of miR-223.

During my post doc I was focused on studying the molecular players and their roles in the regulation of the three-dimensional structure and spatial organization of chromatin via long-range interactions by using cutting edge technology of genome editing and chromatin conformation captures (4C-Hi-C).

As Staff Scientist at the Translational Immunology Section I am developing cutting edge immune monitoring assays essential to determine the immune responses in patients receiving immune therapies. Moreover, I am studying the cellular and molecular target of JAK inhibitors with focus on the epigenetic modifications induced by this class of drugs on immune cells.

Scientific Publications

The Energetics and Physiological Impact of Cohesin Extrusion.

Vian L, Pękowska A, Rao SSP, Kieffer-Kwon KR, Jung S, Baranello L, Huang SC, El Khattabi L, Dose M, Pruett N, Sanborn AL, Canela A, Maman Y, Oksanen A, Resch W, Li X, Lee B, Kovalchuk AL, Tang Z, Nelson S, Di Pierro M, Cheng RR, Machol I, St Hilaire BG, Durand NC, Shamim MS, Stamenova EK, Onuchic JN, Ruan Y, Nussenzweig A, Levens D, Aiden EL, Casellas R
Cell.
2018 Sep 20;
175(1).
doi: 10.1016/j.cell.2018.09.002
PMID: 30241609

A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans.

Tsai PF, Dell'Orso S, Rodriguez J, Vivanco KO, Ko KD, Jiang K, Juan AH, Sarshad AA, Vian L, Tran M, Wangsa D, Wang AH, Perovanovic J, Anastasakis D, Ralston E, Ried T, Sun HW, Hafner M, Larson DR, Sartorelli V
Molecular cell.
2018 Jul 5;
71(1).
doi: 10.1016/j.molcel.2018.06.008
PMID: 29979962

Small molecules to the rescue: Inhibition of cytokine signaling in immune-mediated diseases.

Gadina M, Gazaniga N, Vian L, Furumoto Y
Journal of autoimmunity.
2017 Dec;
85().
doi: 10.1016/j.jaut.2017.06.006
PMID: 28676205

B cell super-enhancers and regulatory clusters recruit AID tumorigenic activity.

Qian J, Wang Q, Dose M, Pruett N, Kieffer-Kwon KR, Resch W, Liang G, Tang Z, Mathé E, Benner C, Dubois W, Nelson S, Vian L, Oliveira TY, Jankovic M, Hakim O, Gazumyan A, Pavri R, Awasthi P, Song B, Liu G, Chen L, Zhu S, Feigenbaum L, Staudt L, Murre C, Ruan Y, Robbiani DF, Pan-Hammarström Q, Nussenzweig MC, Casellas R
Cell.
2014 Dec 18;
159(7).
doi: 10.1016/j.cell.2014.11.013
PMID: 25483777

Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors.

Vian L, Di Carlo M, Pelosi E, Fazi F, Santoro S, Cerio AM, Boe A, Rotilio V, Billi M, Racanicchi S, Testa U, Grignani F, Nervi C
Cell death and differentiation.
2014 Feb;
21(2).
doi: 10.1038/cdd.2013.145
PMID: 24141720

Interactome maps of mouse gene regulatory domains reveal basic principles of transcriptional regulation.

Kieffer-Kwon KR, Tang Z, Mathe E, Qian J, Sung MH, Li G, Resch W, Baek S, Pruett N, Grøntved L, Vian L, Nelson S, Zare H, Hakim O, Reyon D, Yamane A, Nakahashi H, Kovalchuk AL, Zou J, Joung JK, Sartorelli V, Wei CL, Ruan X, Hager GL, Ruan Y, Casellas R
Cell.
2013 Dec 19;
155(7).
doi: 10.1016/j.cell.2013.11.039
PMID: 24360274

A genome-wide map of CTCF multivalency redefines the CTCF code.

Nakahashi H, Kieffer Kwon KR, Resch W, Vian L, Dose M, Stavreva D, Hakim O, Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair RD, Pugh BF, Lobanenkov V, Hager GL, Casellas R
Cell reports.
2013 May 30;
3(5).
doi: 10.1016/j.celrep.2013.04.024
PMID: 23707059

Education

Ph.D. in Cell Science and Morphogenesis Department of Histology and Medical Embryology, "Sapienza", University of Rome (Italy), 2010

Laurea in Genomic Biotechnologies (two-years Master degree). "Sapienza", University of Rome, 2006

Laurea in Biotechnologies. "Sapienza", University of Rome (Italy), 2004

Experience

Staff Scientist (2018 – 2020) National Institute of Arthritis and Musculoskeletal and SkinDiseases (NIAMS), Translational Immunology Section

Research Fellow (2016 – 2018) National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), Translational Immunology Section

Post  Doctoral Fellow (2012 – 2016) National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), Genomics and Immunity Section

Post  Doctoral Fellow (2010 – 2012) “Sapienza” University of Rome, Department of Medico-Surgical Sciences and Biotechnologies, Laboratory of Neoplastic Cell Differentiation

Last Updated: September 2020