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Laboratory of Structural Biology Research
Complete list of publications
Steven AC, Baumeister W. The state of the art. J Struct Biol. 2008 May 20.
Steven AC, Baumeister W. The future is hybrid. J Struct Biol. 2008 Jun 12. [Epub ahead of print]
Uetrecht C, Versluis C, Watts NR, Roos WH, Wuite GJ, Wingfield PT, Steven AC, Heck AJ. High-resolution mass spectrometry of viral assemblies: molecular composition and stability of dimorphic hepatitis B virus capsids. Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9216-20. Epub 2008 Jun 27.Click here to read
Watts NR, Cardone G, Vethanayagam JG, Cheng N, Hultgren C, Stahl SJ, Steven AC, Sällberg M, Wingfield PT. Non-canonical binding of an antibody resembling a naïve B cell receptor immunoglobulin to hepatitis B virus capsids. J Mol Biol. 2008 Jun 20;379(5):1119-29. Epub 2008 Apr 9.Click here to read
Buck CB, Cheng N, Thompson CD, Lowy DR, Steven AC, Schiller JT, Trus BL. Arrangement of L2 within the papillomavirus capsid. J Virol. 2008 Jun;82(11):5190-7. Epub 2008 Mar 26.
Sen A, Heymann JB, Cheng N, Qiao J, Mindich L, Steven AC. Initial location of the RNA-dependent RNA polymerase in the bacteriophage Phi6 procapsid determined by cryo-electron microscopy. J Biol Chem. 2008 May 2;283(18):12227-31. Epub 2008 Feb 20.Click here to read
Uetrecht C, Versluis C, Watts NR, Wingfield PT, Steven AC, Heck AJ. Stability and shape of hepatitis B virus capsids in vacuo. Angew Chem Int Ed Engl. 2008;47(33):6247-51.
Uetrecht C, Versluis C, Watts NR, Wingfield PT, Steven AC, Heck AJ. Inside Cover: Stability and Shape of Hepatitis B Virus Capsids In Vacuo. Angew Chem Int Ed Engl. 2008 Aug 4;47(33):6090.
Sen, A., Baxa, U., Simon, M.N., Wall, J.S., Sabate, R., Saupe, S.J., & Steven, A.C. Mass Analysis by Scanning Transmission Electron Microscopy and Electron Diffraction Validate Predictions of the Stacked ß-Solenoid Model of HET-s Prion Fibrils. J. Biol. Chem. 282, 5545-50 (2007).
Heymann, J. B. and Belnap, D. M. (2007) Bsoft: Image processing and molecular modeling for electron microscopy. J. Struct Biol 157: 3-18.
Cardone, G., Winkler, D.C., Trus, B.L., Cheng, N. Heuser, J.E., Newcomb, W.W., Brown, J.C. & Steven, A.C. Visualization of the Herpes Simplex Virus Portal in situ by Cryo-electron Tomography. Virology. 361(2):426-34 (2007)
Wikoff, W.R., Conway, J.F., Tang, J., Lee, K.K., Gan., L, Cheng, N., Duda, R.L., Hendrix, R.W., Steven, A.C. & Johnson, J.E. Time-resolved Molecular Dynamics of Bacteriophage HK97 Capsid Maturation Interpreted by Electron Cryo-microscopy and X-ray Crystallography. J. Struct. Biol. 153, 300-6 (2006).
Steven, A.C. & Spear, P.G. Viral Glycoproteins and an Evolutionary Conundrum. Science 313, 177-178 (2006).
Ross, P.D., Conway, J.F., Cheng, N., Dierkes, L., Firek, B.A., Hendrix, R.W., Steven, A.C. & Duda, R.L. A Free Energy Cascade with Locks Drives Assembly and Maturation of Bacteriophage HK97 Capsid. J. Mol. Biol. 364, 512-525 (2006).
Kajava, A.V., & Steven, A.C. Beta-Rolls, Beta-Helices and Other Beta-Solenoid Proteins Adv. Prot. Chem. 73, 55-96 (2006).
Kajava, A.V. & Steven, A.C. The Turn of the Screw: Variations of the Abundant ß Solenoid Motif in Passenger Domains of Type V Secretory Proteins . J. Struct. Biol. 155, 306-315 (2006).
Hennetin, J., Jullian, B., Steven, A.C. & Kajava, A.V. Standard Conformations of b -Arches in b -solenoid Proteins J. Mol. Biol. 358, 1094-105 (2006).
Harris, A., Cardone, G., Winkler, D.C., Heymann, J.B., Brecher, M., White, & Steven, A.C. Influenza Virus Pleiomorphy Characterized by Cryo-Electron Tomography. Proc. Nat'l. Acad. Sci. USA 103, 19123-19127(2006).
Harris, A. Belnap, D.M., Watts, N.R., Conway, J.F., Cheng, N., Stahl, S.J., Vethanayagam, J.G., Wingfield, P.T. & Steven, A.C. Epitope Diversity of Hepatitis B Virus Capsids: Quasiequivalent Variations in Spike Epitopes and Binding of Different Antibodies to the Same Epitope. J. Mol. Biol. 355, 562-576 (2006).
Baxa, U., Cassese, T., Kajava, A.V., & Steven, A.C. Structure, Function, and Amyloidogenesis of Fungal Prions: Filament Polymorphism and Prion Variants. Adv. Prot. Chem. 73, 125-80 (2006).
Steven A, Belnap D. Electron microscopy and image processing: an essential tool for structural analysis of macromolecules. Curr Protoc Protein Sci. 2005 Dec;Chapter 17:Unit 17.2
Steven, A.C., Conway, J.F., Cheng, N., Watts, N.R., Belnap, D.M., Harris, A. Stahl, S.J., Wingfield, P.T. Structure, Assembly, and Antigenicity of Hepatitis B Virus Capsid Proteins. Adv. Virus Res. 64, 125-164 (2005).
Steven, A.C. , Heymann, J.B., Cheng, N., Trus, B.L., & Conway , J.F. Virus Maturation: Dynamics and Mechanism of a Stabilizing Structural Transition that Leads to Infectivity. Curr. Opin. Struct. Biol. 15, 27-236 (2005).
Ross, P.D., Cheng, N. Conway , J.F., Firek, B.A., Hendrix, R.W., Duda, R.L., & Steven, A.C. Crosslinking Renders Bacteriophage HK97 Capsid Maturation Irreversible and Effects an Essential Stabilization. EMBO J. 24, 1352-1363 (2005).
Pierce, M.M., Baxa, U., Steven, A.C., Bax, A. & Wickner, R.B. Is The Prion Domain of Soluble Ure2p Unstructured? Biochemistry 44: 321-328 (2005).
Ortega, J., Heymann, J.B., Kajava, A.V., Ustrell, V., Rechsteiner, M., & Steven, A.C. The Axial Channel of the 20S Proteasome Opens upon Binding of the PA200 Activator. J. Mol. Biol. 346, 1221-1227 (2005).
Louis J.M., Byeon I.J., Baxa U. , Gronenborn A.M. The GB1 amyloid fibril: recruitment of the peripheral beta-strands of the domain swapped dimmer into the polymeric interface. J. Mol Biol. 2005 348:687-98.
Li, Y., Conway , J.F., Cheng, N., Steven, A.C., Hendrix, R.W., & Duda, R.L. Control of Virus Assembly: HK97 "Whiffleball" Mutant Capsids Without Pentons. J. Mol. Biol. 348, 167-182 (2005).
Kajava, A.V., Aebi, U., & Steven, A.C. The Parallel Superpleated Beta-structure as a Model for Amyloid Fibrils of Human Amylin. J. Mol. Biol. 348, 247-252 (2005).
Heymann, J.B., Iwasaki, K., Yim, Y-I, Cheng, N. Belnap, D.M., Greene, L.E., Eisenberg, E. & Steven, A.C. Visualization of the Binding of Hsc70 ATPase to Clathrin Baskets: Implications for an Uncoating Mechanism. J.Biol. Chem. 280, 7156-7161 (2005).
Heymann, J. B., Chagoyen, M. and Belnap, D. M. (2005b) Common conventions for interchange and archiving of three-dimensional electron microscopy information in structural biology. J.Struct Biol 151: 196-207.
Fokine, A., Leiman, P.G., Shneider, M.M., Ahvaz, B., Boeshans, K.M., Steven, A.C., Black, L.W., Mesyanzhinov, V.V., & Rossmann, M.G. Structural and Functional Similarities between the Capsid Proteins of Bacteriophages T4 and HK97 Point to a Common Ancestry. Proc. Nat'l. Acad. Sci. USA 102, 7163-7168 (2005).
Davison, A.J., Trus, B.L., Cheng, N., Steven, A.C., Watson, M.S., Cunningham, C., Le Duff, R-M & Renault, T. A Novel Class of Herpesvirus with Bivalve Hosts. J. Gen. Virol. 86, 41-53 (2005).
Cardone, G., Grünewald, K. & Steven, A.C. , A Resolution Criterion for Electron Tomography based on Cross-validation. J. Struct. Biol. 151, 117-129 (2005).
Bubeck, D. Filman, D.J., Cheng, N. Steven, A.C., Hogle, J.M., & Belnap, D.M. Structure of the Poliovirus 135S Cell-Entry Intermediate at 10Å Resolution Reveals the Location of an Externalized Polypeptide that Binds to Membranes. J. Virol. 79, 7745-7755 (2005).
Brachmann A., Baxa U. , Wickner R.B. Prion generation in vitro: amyloid of Ure2p is infectious. EMBO J. 2005 Sep 7;24(17):3082-92. Epub 2005 Aug 11.
Baxa, U., Cheng, N., Winkler, D.C., Chiu, T, Davies, D.R., Sharma, D., Inouye, H., Kirschner, D.A., Wickner, R.B., & Steven, A.C. Filaments of the Ure2p Prion Protein have a Cross-ß Core Structure. J. Struct. Biol. 150, 170-179 (2005).
Trus, B.L., Cheng, N. Newcomb, W.W., Homa, F.L., Brown, J.C., & Steven, A.C. Structure and Polymorphism of the UL6 Portal Protein of Herpes Simplex Virus 1. J. Virol. 78, 12668-71 (2004).
Ross ED, Baxa U, Wickner R.B. Scrambled prion domains form prions and amyloid. Mol Cell Biol.2004 24:7206-13.
Ortega, J., Lee, H-S, Maurizi, M.R., & Steven, A.C. ClpA and ClpX ATPases Bind Simultaneously to Opposite Ends of ClpP Peptidase to Form Active Hybrid Complexes. J. Struct. Biol. 146, 217-226 (2004).
Kalinin , A.E., Idler, W.W., Marekov, L.N., McPhie, P., Bowers, B., Steinert, P.M., & Steven, A.C. Co-assembly of Envoplakin and Periplakin into Oligomers and Ca ++ -dependent Vesicle Binding: Implications for Cornified Cell Envelope Formation in Stratified Squamous Epithelia. J. Biol. Chem. 279, 22773-80 (2004).
Kajava, A.V., Gorbea, C. Ortega, J., Rechsteiner, M. & Steven, A.C. New HEAT-like Repeat Motifs in Proteins Regulating Proteasome Structure and Function. J. Struct. Biol. 146, 425-430 (2004).
Kajava, A.V., Baxa, U., Wickner, R.B., & Steven, A.C. A Model for Ure2p Prion Filaments and Other Amyloids: The Parallel Super-pleated b -Structure. Proc. Nat'l. Acad. Sci. USA 101, 7885-7890.
Ishikawa, T., Maurizi, M.R., & Steven, A.C. , The N-terminal Substrate-Binding Domain of ClpA Unfoldase is Highly Mobile and Extends Axially from the Distal Surface of ClpAP Protease. J. Struct. Biol. 146, 180-188 (2004).
Ishikawa, T., Cheng, N., Liu, X., Korn, E.D., & Steven, A.C. Subdomain Organization of the Acanthamoeba myosin IC Tail from Cryo-electron Microscopy. Proc. Nat'l Acad. Sci. US 101, 12189-12194 (2004).
Heyman, J.B., Conway , J.F., & Steven, A.C. Molecular Dynamics of Protein Complexes from Four- dimensional Cryo-Electron Microscopy. J. Struct. Biol. 147, 291-301 (2004).
Gan, L., Conway, J.F., Firek, Cheng, Naiqian, Hendrix, R.W., Steven, A.C., Johnson, J.E. & Duda, R.L. Control of Cross-linking by Quaternary Structure Changes During Bacteriophage HK97 Maturation. Mol. Cell 14, 559-69 (2004).
Baxa, U., Ross, P.D., Wickner, & Steven A.C. The N-terminal Prion Domain of Ure2p Converts from an Unfolded to a Thermally Resistant Conformation upon Filament Formation. J. Mol. Biol. 339, 259-264 (2004).
Baxa, U, Taylor , K.L., Steven, A.C. , & Wickner, R.B. Prions of Saccharomyces and Podospora . in “Prions: A Challenge for Science, Medicine and Public Health Systems” (Eds. H.F. Rabenau, J. Cinari, H.W. Doerr). S. Karger AG, Basel pp. 50-71 (2004). Prions of Saccharomyces and Podospora.
Grunewald K, Desai P, Winkler DC, Heymann JB, Belnap DM, Baumeister W, Steven AC. Three-dimensional structure of herpes simplex virus from cryo-electron tomography. Science. 2003; 302(5649): 1396-8.
Baxa U, Taylor KL, Wall JS, Simon MN, Cheng N, Wickner RB, Steven AC. Architecture of Ure2p prion filaments: the N-terminal domains form a central core fiber. J Biol Chem. 2003; 278(44): 43717-27.
Belnap DM, Watts NR, Conway JF, Cheng N, Stahl SJ, Wingfield PT, Steven AC. Diversity of core antigen epitopes of hepatitis B virus. Proc Natl Acad Sci U S A. 2003; 100(19):10884-9.
Conway JF, Watts NR, Belnap DM, Cheng N, Stahl SJ, Wingfield PT, Steven AC. Characterization of a conformational epitope on hepatitis B virus core antigen and quasiequivalent variations in antibody binding. J Virol. 2003; 77(11): 6466-73.
Heymann JB, Cheng N, Newcomb WW, Trus BL, Brown JC, Steven AC. Dynamics of herpes simplex virus capsid maturation visualized by time-lapse cryo-electron microscopy. Nat Struct Biol. 2003; 10(5):334-41.
Cerritelli ME, Trus BL, Smith CS, Cheng N, Conway JF, Steven AC. A second symmetry mismatch at the portal vertex of bacteriophage T7: 8-fold symmetry in the procapsid core. J Mol Biol. 2003; 327(1):1-6.
Steven AC, Aebi U. The next ice age: cryo-electron tomography of intact cells. Trends Cell Biol. 2003; 13(3):107-10.
Freiberg A, van den Bosch L, Morona R, Jung C, Behlke J, Carlin N, Seckler R, Baxa U. The tailspike protein of Shigella phage Sf6: A structural homolog of Salmonella phage P22 tailspike protein without sequence similarity in the βhelix domain. J. Biol Chem. 2003; 278(3): 1542-8.
Tang SX, Murakami T, Cheng NQ, Steven AC, Freed EO, Levin JG. Human immunodeficiency virus type 1 N-terminal capsid mutants containing cores with abnormally high levels of capsid protein and virtually no reverse transcriptase. Journal of Virology 2003; 77(23): 12592-602
Heymann, J.B., Cheng, N., Newcomb, W.W., Trus, B.L., Brown, J.C. & Steven, A.C. Dynamics of Herpes Simplex Virus Capsid Maturation Visualized by Time-Lapsed Cryo-EM. Nature Struct. Biol. 10, 334-341 (2003).
Grunewald K, Medalia O, Gross A, Steven AC, Baumeister W. Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments: implications of crowding. Biophys Chem. 2003; 100(1-3): 577-91.
Cerritelli, M.E., Conway, J.F., Cheng, N., Trus, B.L. & Steven, A.C. Molecular Mechanisms in Bacteriophage T7 Procapsid Assembly and Maturation in Adv. Prot. Chem. Vol. 64, Virus Structure (W. Chiu, J.E. Johnson, eds.) Academic Press. pp 301-324 (2003).
Cheng N, Trus BL, Belnap DM, Newcomb WW, Brown JC, Steven AC. Handedness of the herpes simplex virus capsid and procapsid. J Virol. 2002; 76(15):7855-9.
Baxa U, Speransky V, Steven AC, Wickner RB. Mechanism of inactivation on prion conversion of the Saccharomyces cerevisiae Ure2 protein. Proc Natl Acad Sci U S A. 2002; 99(8):5253-60.
Jarnik M, de Viragh PA, Scharer E, Bundman D, Simon MN, Roop DR, Steven AC. Quasi-normal cornified cell envelopes in loricrin knockout mice imply the existence of a loricrin backup system. J Invest Dermatol. 2002; 118(1):102-9.
Zhang, A., Wassarman, K.M., Ortega, J., Steven, A.C. & Storz, G. The Sm-like Hfq protein increases OxyS sRNA interaction with target mRNAs. Mol. Cell 9: 11-22 (2002).
Zakalskiy, A., Högenauer, G., Ishikawa, T., Wehrschütz-Sigl, E., Wendler, F., Teis, D., Zisser, G., Steven, A.C. and Bergler, H. Structural and Enzymatic Properties of the AAA-protein Drg1p from Saccharomyces cerevisiae : Decoupling of Intracellular Function from ATPase Activity and Hexamerization. J. Biol. Chem. 277, 26788-27895 (2002).
Watts, N.R., Conway , J.F., Cheng, N., Stahl, S.J., Belnap, D.M., Steven, A.C. & Wingfield, P.T. The Morphogenic Linker Peptide of HBV Capsid Protein Forms a Mobile Array on the Interior Surface. EMBO J. 21: 876-884 (2002).
Watts , N.R., Jones, L.N., Cheng, N., Wall, J.S., Parry, D.A.D., Steven, A.C., Cryo-Electron Microscopy of Trichocyte (Hard Alpha-Keratin). Intermediate Filaments Reveals a Low-Density Core. J. Struct. Biol. 137:109-18 (2002).
Watts , N.R., Cheng, N. West, W., Steven, A.C. & Sackett, D.L. Cryptophycin-Tubulin Ring Structure Indicates Two Points of Curvature in the Tubulin Ring. Biochemistry 41, 12662-12669 (2002).
Ortega, J., Lee, H-S., Maurizi, M.R., & Steven, A.C. Alternating Translocation of Protein Substrates from Both Ends of ClpXP Protease. EMBO J. 21, 4938-4949 (2002).
Kang, S.Gy., Ortega, J., Singh, S.K., Wang, N., Huang, N., Steven, A.C. & M.R. Maurizi. Functional Proteolytic Complexes of the Human Mitochondrial ATP-dependent Protease, hClpXP. J. Biol. Chem. 277, 21095-21102 (2002).
Fernández-Salas, E., Suh, K.S., Speransky, V.V., Bowers, W.L., Levy, J.M., Adams, T. Pathak, K. R., Edwards, L.E., Hayes, D.D., Cheng, C. Steven, A.C., Weinberg,W.C. & Yuspa, S.H. MtCLIC/CLIC4, an Organellular chloride Channel Protein, is Increased by DNA Damage and Participates in the Apoptotic Response to p53. Mol. Cell Biol. 22, 3610-3620 (2002).
Barrientos, L.G., Gawrisch, K., Cheng, N., Steven, A.C. & Gronenbom. Structural Characterization of the Dilute Aqueous Surfactant Solution of Cetylpyridinium Bromide/Hexanol/Sodium Bromide. Langmuir 18: 3773-3779 (2002).
Trus, B.L., Heymann, J.B., Nealon, K., Cheng, N., Newcomb, W.W., Brown, J.C., Kedes, D.H., Steven, A.C. Capsid Structure of Kaposi's Sarcoma-Associated Herpesvirus, a Gammaherpesvirus, Compared to an Alphaherpesvirus, HSV1, and a Betaherpesvirus, CMV J. Virol. 75, 2879-2890 (2001).
Updated September 17, 2007
