Research

Photo of Confocal Microscope

Protein Expression Laboratory

Extended list of publications

Fang X, Wang J, O'Carroll IP, Mitchell M, Zuo X, Wang Y, Yu P, Liu Y, Rausch JW, Dyba MA, Kjems J, Schwieters CD, Seifert S, Winans RE, Watts NR, Stahl SJ, Wingfield PT, Byrd RA, Le Grice SF, Rein A, Wang YX. An unusual topological structure of the HIV-1 Rev response element. Cell. 2013 Oct 24;155(3):594-605. doi: 10.1016/j.cell.2013.10.008. PubMed Icon

Bereszczak JZ, Rose RJ, van Duijn E, Watts NR, Wingfield PT, Steven AC, Heck AJ. Epitope-distal Effects Accompany the Binding of Two Distinct Antibodies to Hepatitis B Virus Capsids. J Am Chem Soc. 2013 May 1;135(17):6504-12. doi: 10.1021/ja402023x. PubMed Icon

Watts NR, Conway JF, Cheng N, Stahl SJ, Steven AC, Wingfield PT. Role of the Propeptide in Controlling Conformation and Assembly State of Hepatitis B Virus e-Antigen. J Mol Biol. 2011 Apr 2. [Epub ahead of print] PubMed Icon

Uetrecht C, Watts NR, Stahl SJ, Wingfield PT, Steven AC, Heck AJ. Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent. Phys Chem Chem Phys. 2010 Nov 7;12(41):13368-71. PubMed Icon

Daniels SI, Davis DA, Soule EE, Stahl SJ, Tebbs IR, Wingfield P, Yarchoan R. The initial step in human immunodeficiency virus type 1 GagProPol processing can be regulated by reversible oxidation. PLoS One. 2010 Oct 22;5(10):e13595. PubMed Icon

Roos WH, Gibbons MM, Arkhipov A, Uetrecht C, Watts NR, Wingfield PT, Steven AC, Heck AJ, Schulten K, Klug WS, Wuite GJ. Squeezing protein shells: how continuum elastic models, molecular dynamics simulations, and experiments coalesce at the nanoscale. Biophys J. 2010 Aug 9;99(4):1175-81. PubMed Icon

Watts NR, Vethanayagam JG, Ferns RB, Tedder RS, Harris A, Stahl SJ, Steven AC, Wingfield PT. Molecular basis for the high degree of antigenic cross-reactivity between hepatitis B virus capsids (HBcAg) and dimeric capsid-related protein (HBeAg): insights into the enigmatic nature of the e-antigen. J Mol Biol. 2010 May 14;398(4):530-41. PubMed Icon

Stahl SJ, Watts NR, Rader C, DiMattia MA, Mage RG, Palmer I, Kaufman JD, Grimes JM, Stuart DI, Steven AC, Wingfield PT. Generation and characterization of a chimeric rabbit/human Fab for co-crystallization of HIV-1 Rev. J Mol Biol. 2010 Apr 2;397(3):697-708. PubMed Icon

DiMattia MA, Watts NR, Stahl SJ, Rader C, Wingfield PT, Stuart DI, Steven AC, Grimes JM. Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element. Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5810-4. PubMed Icon

Jedidi I, Zhang F, Qiu H, Stahl SJ, Palmer I, Kaufman JD, Nadaud PS, Mukherjee S, Wingfield PT, Jaroniec CP, Hinnebusch AG. Activator Gcn4 employs multiple segments of Med15/Gal11, including the KIX domain, to recruit mediator to target genes in vivo. J Biol Chem. 2010 Jan 22;285(4):2438-55. PubMed Icon

Dolinska MB, Sergeev YV, Chan MP, Palmer I, Wingfield PT. N-terminal extension of beta B1-crystallin: identification of a critical region that modulates protein interaction with beta A3-crystallin. Biochemistry. 2009 Oct 13;48(40):9684-95. PubMed Icon

Davis DA, Tebbs IR, Daniels SI, Stahl SJ, Kaufman JD, Wingfield P, Bowman MJ, Chmielewski J, Yarchoan R. Analysis and characterization of dimerization inhibition of a multi-drug-resistant human immunodeficiency virus type 1 protease using a novel size-exclusion chromatographic approach. Biochem J. 2009 Apr 15;419(2):497-506. PubMed Icon

Chan MP, Dolinska M, Sergeev YV, Wingfield PT, Hejtmancik JF. Association properties of betaB1- and betaA3-crystallins: ability to form heterotetramers. Biochemistry. 2008 Oct 21;47(42):11062-9 PubMed Icon

Watts NR, Cardone G, Vethanayagam JG, Cheng N, Hultgren C, Stahl SJ, Steven AC, Sällberg M, Wingfield PT. Non-canonical binding of an antibody resembling a naïve B cell receptor immunoglobulin to hepatitis B virus capsids. J Mol Biol. 2008 Jun 20;379(5):1119-29. PubMed Icon

Uetrecht C, Versluis C, Watts NR, Wingfield PT, Steven AC, Heck AJ. Stability and shape of hepatitis B virus capsids in vacuo. Angew Chem Int Ed Engl. 2008;47(33):6247-51. PubMed Icon

Davis DA, Brown CA, Singer KE, Wang V, Kaufman J, Stahl SJ, Wingfield P, Maeda K, Harada S, Yoshimura K, Kosalaraksa P, Mitsuya H, Yarchoan R. Inhibition of HIV-1 replication by a peptide dimerization inhibitor of HIV-1 protease. Antiviral Res. 2006 Nov;72(2):89-99. PubMed Icon

Conway JF, Watts NR, Belnap DM, Cheng N, Stahl SJ, Wingfield PT, Steven AC. Characterization of a conformational epitope on hepatitis B virus core antigen and quasiequivalent variations in antibody binding. J Virol. 2003; 77(11): 6466-73. PubMed Icon

Watts NR, Sackett DL, Ward R, Miller M, Stahl SJ, Trus BL, Wingfield PT, Steven AC. Interaction of HIV-l Rev with Tubulin. J CelI Biol. 2000; 150: 349-360. PubMed Icon

Davis DA, Newcomb FM, Moskovitz J, Wingfield PT, Stahl SJ, Kaufmann S, et al. HIV-2 Protease is inactivated following oxidation at the dimer interface and activity can be restored with methionine sulfoxide reductase. Biochem J. 2000; 346: 305-11. PubMed Icon

Wang Y-X, Jacob J, Neamati N, Palmer I, Stahl SJ, Kaufman SD, et al. Solution structure of anti-HIV-l and anti-tumor protein MAP3O: Structural insights into its multiple functions. Cell. 1999; 99: 433-42. PubMed Icon

Zlotnick A, Johnson JM, Wingfield PT, Stahl SJ, Endres D. A theoretical model successfully identifies features of Hepatitis B capsid assembly. Biochemistry. 1999; 38: 14644-52. PubMed Icon

Caffrey M, Kaufman J, Stahl S, Wingfield P, Gronenborn AM, Clore GM. Monomer-turner equilibrium of the HIV gp4l ectodomain: insight into the mechanism of peptide inhibition of SIV infection. Protein Sci. 1999; 8: 1904-07. PubMed Icon

Yang Z-N, Mueser TC, Kaufman J, Stahl SJ, Wingfield PT, Hyde C. The crystal structure of the HIV gp4l ectodomain at 1.47A resolution. J Struct Biol. 1999; 126: 133-144. PubMed Icon

Caffrey M, Kaufman J, Stahl SJ, Wingfield PT, Gronenborn A, Clore AM. Three-dimensional NMR experiments for measuring 15N relaxation data of large proteins: application to the 44 kDa ectodomain of SIV gp41. J Magn Reson. 1998; 135: 368-72. PubMed Icon

Caffrey M, Cai M, Kaufman J, Stahl S, Wingfield PT, Covell DG, et al. Three-dimensional solution structure of the 44 kDa ectodomain of SIV gp41. EMBO J. 1998; 17:4572-84. PubMed Icon

Watts NR, Misra M, Wingfield PT, Stahl SJ, Cheng N, Trus BL, Steven AC, Williams RW. Three-dimensional structure of HIV-1 Rev protein filaments. J Struct Biol. 1998; 121(1): 41-52. PubMed Icon

Wingfield PT, Stahl SJ, Kaufman J, Zlotnick A, Hyde CC, Gronenborn AM, Clore GM. The extracellular domain of immunodeficiency virus gp4l protein: expression in Escherichia coli, purification and crystallization. Protein Sci. 1997; 6: 1653-1660. PubMed Icon

Wingfield PT, Stahl SJ, Payton MA, Venkatesan S, Misa M, Steven A. HIV-1 Rev expressed in recombinant Escherichia coli: purification, polymerization and conformational properties. Biochemistry 1991; 30: 7527-7534. PubMed Icon

 

Updated January 13, 2014